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gad-public
singularity
Commits
6817ed5c
Commit
6817ed5c
authored
Jun 18, 2024
by
Valentin Vautrot
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Install GD perl module for bismark Mbiais plots
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7568d221
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bismark_0.24.2.def
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bismark_0.24.2.def
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6817ed5c
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@@ -25,11 +25,30 @@ apt-get install -y --no-install-recommends \
samtools \
python2.7 \
python2.7-dev \
perl \
libtbb2
# hisat2
ln -s /usr/bin/python2.7 /usr/bin/python
# install perl and required librairies and tools
apt-get install -y --no-upgrade --no-install-recommends \
perl \
build-essential \
cpanminus \
pkg-config \
libgd3 \
libgd-dev \
libpng16-16 \
libimage-png-libpng-perl \
libfont-freetype-perl
# install GD for perl
cpan -v
cpanm -v
cpan App::cpanminus
cpanm ExtUtils::PkgConfig
cpanm GD
cpanm GD::Graph
# get bismark code (no repo)
wget -P /usr/bin/bismark --no-check-certificate https://github.com/FelixKrueger/Bismark/archive/refs/tags/v0.24.2.tar.gz
tar -xvzf /usr/bin/bismark/v0.24.2.tar.gz -C /usr/bin/
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@@ -38,6 +57,7 @@ rm -rf /usr/bin/bismark/
# create mount directory for bowtie2 bind, to avoid problems
mkdir /usr/bin/bowtie2
%environment
export PATH=${PATH}:/usr/bin/samtools/:/usr/bin/Bismark-0.24.2/:/usr/bin/bowtie2/:/usr/bin/python:/usr/bin/python2.7:/usr/lib/x86_64-linux-gnu/:/usr/lib/python2.7/
export PYTHON=/usr/bin/python
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