#! /bin/sh ### ASDP PIPELINE ### ## Version: 0.0.1 ## Licence: AGPLv3 ## Author: anne-sophie.denomme-pichon@u-bourgogne.fr ## Description: a wrapper for qsubing ExpansionHunter denovo script for STR detection ## Usage: qsub -pe smp 1 -v INPUTFILE=<path to the bam file>,OUTPUTPREFIX=<output prefix>,[LOGFILE=<path to the log file>] wrapper_ehdn.sh # Log file path option if [ -z "$LOGFILE" ] then LOGFILE=ehdn.$(date +"%F_%H-%M-%S").log fi # Logging exec 1>> "$LOGFILE" 2>&1 echo "$(date +"%F_%H-%M-%S"): START" # Check if input file exists if [ ! -f "$INPUTFILE" ] then echo "Input file '$INPUTFILE' does not exist" echo "$(date +"%F_%H-%M-%S"): END" exit 1 fi # Check if output prefix is specified if [ -z "$OUTPUTPREFIX" ] then echo "Output prefix is not specified" echo "$(date +"%F_%H-%M-%S"): END" exit 1 fi # Create .bam and .bai symbolic links TMPDIR="$(mktemp -d)" ln -s "$INPUTFILE" "$TMPDIR/$(basename "$INPUTFILE")" ln -s "$(echo "$INPUTFILE" | sed 's/\.bam$/.bai/')" "$TMPDIR/$(basename "$INPUTFILE").bai" # Launch script command and check exit code echo "command : /work/gad/shared/bin/expansionhunterdenovo/ExpansionHunterDenovo-v0.8.0-linux_x86_64/bin/ExpansionHunterDenovo-v0.8.0 profile \ --reads "$TMPDIR/$(basename "$INPUTFILE")" \ --reference /work/gad/shared/pipeline/hg19/index/hg19_essential.fa \ --output-prefix $OUTPUTPREFIX \ --min-anchor-mapq 50 \ --max-irr-mapq 40" /work/gad/shared/bin/expansionhunterdenovo/ExpansionHunterDenovo-v0.8.0-linux_x86_64/bin/ExpansionHunterDenovo-v0.8.0 profile \ --reads "$TMPDIR/$(basename "$INPUTFILE")" \ --reference /work/gad/shared/pipeline/hg19/index/hg19_essential.fa \ --output-prefix "$OUTPUTPREFIX" \ --min-anchor-mapq 50 \ --max-irr-mapq 40 ehdn_exitcode=$? # Remove .bam and .bai symbolic links rm "$TMPDIR/$(basename "$INPUTFILE")" rm "$TMPDIR/$(basename "$INPUTFILE").bai" rmdir "$TMPDIR" echo "ehdn exit code : $ehdn_exitcode" if [ $ehdn_exitcode != 0 ] then echo "$(date +"%F_%H-%M-%S"): END" exit 1 fi echo "$(date +"%F_%H-%M-%S"): END"